"""This script is supposed to be launched from fiji's jython interpreter """ # # note: fiji's jython doesn't support encoding keyword # https://imagej.net/Scripting_Headless #@ String raw_images_root_path import unittest # unittest2 doesn't exist in fiji import sys from lipase.imageengine import IImageEngine, PixelType, Aabb, NullDebugger, FileBasedDebugger from lipase.imagej.ijimageengine import IJImageEngine, IJImage from lipase.telemos import WhiteEstimator, correct_non_uniform_lighting from lipase.maxima_finder import MaximaFinder from lipase.template_matcher import TemplateMatcher from lipase.traps_detector import TrapsDetector from lipase.catalog import ImageCatalog, Sequence from lipase.lipase import Lipase, ImageLogger from lipase.lipase import GlobulesAreaEstimator, EmptyFrameBackgroundEstimator from lipase.circsymdetector import CircularSymmetryDetector from lipase.imagej.hdf5serializer import save_hdf5_file class TestLipase(unittest.TestCase): RAW_IMAGES_ROOT_PATH = raw_images_root_path # eg '/Users/graffy/ownCloud/ipr/lipase/raw-images' pylint: disable=undefined-variable # we need to know if the test succeeded or not https://stackoverflow.com/questions/4414234/getting-pythons-unittest-results-in-a-teardown-method # CURRENT_RESULT = None # holds last result object passed to run method def setUp(self): print("initializing TestLipase instance") IImageEngine.set_instance(IJImageEngine(debugger=FileBasedDebugger('./debug-images'))) self.catalog = ImageCatalog(self.RAW_IMAGES_ROOT_PATH) def tearDown(self): print("uninitializing TestLipase instance") self.catalog = None # def test_estimate_white(self): # sequence = self.catalog.sequences['res_soleil2018/GGH/GGH_2018_cin2_phiG_I_327_vis_-40_1/Pos2'] # white_estimator = WhiteEstimator(open_size=75, close_size=75, average_size=75) # white_estimate = white_estimator.estimate_white([sequence], ['DM300_327-353_fluo']) # # find_white_reference_image(white_estimate, sequence.get_white()) # print(white_estimate) # IImageEngine.get_instance().debugger.on_image(white_estimate, 'white_estimate') # # assert False, "hellooooo" # print('end of test_estimate_white') # def test_uniform_lighting_correction(self): # non_uniform_sequence = self.catalog.sequences['res_soleil2018/GGH/GGH_2018_cin2_phiG_I_327_vis_-40_1/Pos0'] # uniform_sequence = correct_non_uniform_lighting(non_uniform_sequence, 'DM300_nofilter_vis', white_estimator=WhiteEstimator(open_size=75, close_size=75, average_size=75)) # pylint: disable=unused-variable # def test_template_matcher(self): # sequence = self.catalog.sequences['res_soleil2018/GGH/GGH_2018_cin2_phiG_I_327_vis_-40_1/Pos0'] # stack = sequence.as_hyperstack(['DM300_nofilter_vis'], selected_frames=[0]) # first_image = stack.get_image(frame_index=0) # x_min = 423 # x_max = 553 # y_min = 419 # y_max = 533 # template_trap_aabb = Aabb(x_min, y_min, x_max, y_max) # template_trap_image = first_image.get_subimage(template_trap_aabb) # for image in [first_image, template_trap_image]: # print(image.get_pixel_type(), image.get_width(), image.get_height()) # # the typical value of peaks is -2.e10 and the value between peaks is below -8.0e10 # threshold = -3.0e10 # tolerance = 1.0e10 # maxima_finder = MaximaFinder(threshold, tolerance) # template_matcher = TemplateMatcher(maxima_finder) # matches = template_matcher.match_template(first_image, template_trap_image) # num_traps = len(matches) # print("number of traps found : %d" % num_traps) # num_expected_traps = 13 # 13 traps are completely visible in the first image # self.assertAlmostEqual(len(matches), num_expected_traps, delta=1.0) # def test_traps_detector(self): # # the typical value of peaks is -500 and the value between peaks is below -2500 # threshold = -1500.0 # tolerance = 1500 # maxima_finder = MaximaFinder(threshold, tolerance) # template_matcher = TemplateMatcher(maxima_finder) # traps_detector = TrapsDetector(template_matcher) # sequence = self.catalog.sequences['res_soleil2018/GGH/GGH_2018_cin2_phiG_I_327_vis_-40_1/Pos0'] # x_min = 423 # x_max = 553 # y_min = 419 # y_max = 533 # trap_aabb = Aabb(x_min, y_min, x_max, y_max) # traps_mask = traps_detector.compute_traps_mask(sequence, 'DM300_nofilter_vis', trap_aabb) # measured_mean_value = traps_mask.get_mean_value() # expected_traps_coverage = 0.07909 # traps_pixel_value = 255.0 # expected_mean_value = expected_traps_coverage * traps_pixel_value # print("expected_mean_value: %f" % expected_mean_value) # print("measured_mean_value: %f" % measured_mean_value) # self.assertAlmostEqual(measured_mean_value, expected_mean_value, delta=0.01) # def test_visible_traps_sequence_processing(self): # traps_sequence = self.catalog.sequences['res_soleil2018/GGH/GGH_2018_cin2_phiG_I_327_vis_-40_1/Pos0'] # visible_traps_sequence = traps_sequence.as_hyperstack(['DM300_nofilter_vis']) # background_estimator = EmptyFrameBackgroundEstimator(empty_frame_index=39) # processor = GlobulesAreaEstimator(background_estimator=background_estimator, particle_threshold=2000.0) # results = processor.detect_particles(visible_traps_sequence) # save_hdf5_file('results.h5', results) # # results file could be checked with "h5dump --xml ./lipase.git/results.h5" # first_frame_measured_ratio = results['globules_area_ratio'][(0,)] # first_frame_expected_ratio = 0.008 # self.assertAlmostEqual(first_frame_measured_ratio, first_frame_expected_ratio, delta=0.01) def test_circle_detector(self): traps_sequence = self.catalog.sequences['res_soleil2018/GGH/GGH_2018_cin2_phiG_I_327_vis_-40_1/Pos0'] visible_traps_sequence = traps_sequence.as_hyperstack(['DM300_nofilter_vis']) src_image = visible_traps_sequence.get_image(frame_index=0) ie = IImageEngine.get_instance() detector = CircularSymmetryDetector(max_radius=10.0) radial_profiles = detector.compute_radial_profiles(src_image) # def test_lipase_process(self): # lipase = Lipase(self.catalog, debugger=NullDebugger()) def run_script(): # unittest.main() # this would result in : ImportError: No module named __main__ # solution from : https://discourse.mcneel.com/t/using-unittest-in-rhino-python-not-possible/15364 suite = unittest.TestLoader().loadTestsFromTestCase(TestLipase) stream = sys.stdout # by default it's sys.stderr, which doesn't appear in imagej's output test_result = unittest.TextTestRunner(stream=stream, verbosity=2).run(suite) print('test_result : %s' % test_result) # store summary of the result in a file so that the caller of imagej can detect that this python script failed (imagej seems to always return error code 0, regardless the error returned by the python script it executes : even sys.exit(1) doesn't change this) with open('/tmp/test_result.txt', 'w') as f: f.write('%d' % {True: 0, False: 1}[test_result.wasSuccessful()]) print('end of run_script') # note : when launched from fiji, __name__ doesn't have the value "__main__", as when launched from python run_script()